Genome sequencing, annotation and analysis of Salmonella enterica sub species salamae strain DMA-1

نویسندگان

  • Sathyaseelan Sathyabama
  • Gurwinder Kaur
  • Amit Arora
  • Sheenam Verma
  • Nida Mubin
  • Shanmugam Mayilraj
  • Javed N Agrewala
چکیده

BACKGROUND The genus Salmonella is Gram-negative which belongs to the family Enterobacteriaceae. In this study, we have sequenced the whole genome of the strain DMA-1, which was isolated from mouse stool sample and identified as Salmonella enterica subspecies salamae. RESULTS The strain DMA-1 was closely related at the 16S rRNA gene sequence level with the members of the genus Salmonella: Salmonella enterica subspecies salamae DSM 9220T (100%), followed by Salmonella enterica subspecies diarizonae (99.1%), Salmonella enterica subspecies enterica (99.0%) and Salmonella enterica subspecies indica (98.5%). We obtained the draft genome of S. enterica subspecies salamae strain DMA-1 with a size of 4,826,209 bp and mean G+C content of 52.0 mol%. CONCLUSIONS We for the first time, sequenced the entire genome of the strain DMA-1 which was isolated from the mouse stool sample and identified it as Salmonella enterica, sub species salamae. Further, we subjected the whole genome sequencing data for annotation that revealed several genes responsible for the pathogenesis, virulence, defense, metabolism and other genomic features.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

The Type III Secretion System Effector SeoC of Salmonella enterica subsp. salamae and S. enterica subsp. arizonae ADP-Ribosylates Src and Inhibits Opsonophagocytosis

Salmonella species utilize type III secretion systems (T3SSs) to translocate effectors into the cytosol of mammalian host cells, subverting cell signaling and facilitating the onset of gastroenteritis. In this study, we compared a draft genome assembly of Salmonella enterica subsp. salamae strain 3588/07 against the genomes of S. enterica subsp. enterica serovar Typhimurium strain LT2 and Salmo...

متن کامل

Complete Genome Sequence of Bacteriophage SEN8, a Temperate Phage Isolated from Salmonella enterica subsp. salamae

A temperate phage, SEN8, having a broad activity against pathogenic Salmonella serovars, was isolated from Salmonella enterica subsp. salamae strain Sen8. The complete genome sequence of phage SEN8 was determined (35,203 bp) and showed relatedness to P2-like phages (Salmonella phages Fels-2 and RE-2010).

متن کامل

Whole-Genome Sequence and Annotation of Salmonella enterica subsp. enterica Serovar Enteritidis Phage Type 8 Strain EN1660

The genome of Salmonella enterica subspecies enterica serovar Enteritidis phage type 8 strain EN1660, isolated from an outbreak in Thunder Bay, Canada, was sequenced to 46-fold coverage using an Illumina MiSeq with 300-bp paired-end sequencing chemistry to produce 28 contigs with an N50 value of 490,721 bp.

متن کامل

Complete Genome Sequence of Salmonella enterica subsp. enterica Serovar Thompson Strain RM6836

Salmonella enterica subsp. enterica serovar Thompson strain RM6836 was isolated from lettuce in 2002. We report here the complete sequence and annotation of the genome of S. Thompson RM6836. This is the first reported complete genome sequence for S. Thompson and it will enhance our understanding of this serovar and provide another point for comparative studies between Salmonella enterica strains.

متن کامل

Prevalence and characteristics of Salmonella species isolated from captive reptiles in the Czech Republic

This study was aimed at determining the prevalence and characterising the strains of Salmonella species in various captive reptiles in the Czech Republic. A total of 211 samples of cloacal swabs from lizards, chelonians and snakes, and 14 swabs from terraria surfaces were collected between November 2014 and July 2015. After isolation according to the reference method (EN ISO), Salmonella spp. i...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره 6  شماره 

صفحات  -

تاریخ انتشار 2014